The cytological studies are important in plant species and their population specifically wild and endemic species is significant, because it could be explained genetically variation, variation in their shapes and the size of chromosome in the mitosis division and the behaviour of chromosomes in the meiotic division. Therefore, studies on karyotype have a well-known importance for species characterization, which has a great value because the chromosomal formula is useful to prove taxonomic place of each species, to choose the best way for choice and to understand speciation process. Also, Karyotype describe the phenotypic features, the chromosomes complement of species in their terms of number, size, arm ratio and centromere index and other landmark. Karyotype dynamic structure evolving through numerical and structural changes. The difference in the number of chromosomes cause of characteristic creation and separation and therefore, karyotype evolution is significant important. In addition, for several decades, Karyotype diversity has been a crux of plant evolution studies for the main reasons.