The dry shaded Nicotianatabacum leaves were collected and taken into the dry round bottom flask. Methanol was added into the round bottom flask. The sample was heated for 3 hours at 40ºC in a mandle. The mother -liquid of the sample was collected in a beaker through what man filter paper. The sample was filtered and concentrated. Then it is allowed to cool, storied in a container and kept in freezer for further investigation. GC-MS analysis Gas Chromatography Mass Spectrometry (GC/MS) is an instrumental technique, comprising a gas chromatograph (GC) coupled to a mass spectrometer (MS), by which complex mixtures of chemicals may be separated, identified and quantified. GC-MS analysis of extract was carried out on Gas Chromatograph interfaced to a Mass Spectrometer equipped with a DB-5 MS capillary standard non-polar column of 30 m length.