Quorum sensing is a cell-to-cell signaling process that allows bacteria to collectively control gene expression, thereby harmonizing activities that are productive only at a high population density such as biofilm formation and virulence factors production. This process is accomplished through the production, secretion, and detection of small chemical signals called autoinducers. Autoinducer-2 kinase (LsrK) is the kinase protein present in the quorum sensing system. Generally; kinase protein has been implicated in the progression of a wide range of diseases, making these signaling molecules key therapeutic targets for pharmaceutical development. Hence, we engrossed on LsrK; Gene was cloned into pET30b (+) with specific Denatured LsrK was obtained after treatment GnHCl. These efficient and optimized methods were supportive for refolding and crystallization.