A crucial survival factor for leading pathogen (carrying 40-60% mortality rate) is Tetrahydrodipicolinate NSuccinyltransferase (DAPD EC 2.3.1.117) involves in lysine biosynthesis pathway of P. aeruginosa. Targeting the lysine biosynthesis pathway is a opportunistic and logical site to inhibit the effect of this organism. Withania somnifera, also known as “Indian ginseng”, a reputed herb in ayurvedic medicine, constituting various steroidal lactones (withanolides, withaferins) and saponins which shown various pharmacological activities. We described the docking of 11 secondary metabolites from Withania somnifera into the three dimensional structure of DapD protein of P. aeruginosa using swiss-dock server. Simultaneously, we have checked the ADME values and pharmacokinetics values of these secondary metabolites against the target protein. We have followed the “Lipnski rule of 5” principle and “Molinspiration” tool. Dihydowithaferin, 4-B, Hydroxywithanolide, Withanoloide E, Withanoloide F, Withanoloide D, Withanoloide A) have potential to be used as medicine to treat P.aeruginosa infections. Several known antibiotics also studied along with this approach for comparison purpose.